A histidine-tagged cell-wall-involved protein from Staphylococcus aureus was expressed in Lactococcus lactis and purified working with His Mag Sepharose® Ni. The first goal was to get higher purity, hence 50 mM imidazole was A part of the sample and binding buffer. Fig. one: Workflow of a normal protein purification treatment https://lingjunbio.quora.com/Lnjnbio-Magnetic-Beads-for-DNA-RNA-Extraction-and-Purification-of-Virus-160013-Home-https-www-lingjunbio-com-Pro